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What happens in the laboratory? the time between the puncture and the replacement of the embryos?

Day 0 after the puncture
All usable eggs are extracted from the patient's ovaries (follicle puncture). This procedure can be carried out under a light anaesthetic or general anaesthesia (performed by an anaesthetist). The patient will be asked to empty her bladder before the procedure. The puncture is followed by the fertilisation using IVF or ICSI. In IVF, eggs and sperm are concentrated in vitro. In ICSI, one single sperm is inserted into the egg using a hollow needle.

Day 1 after the puncture
Some eggs will fertilise and others won’t. The fertilised eggs are recognisable because they have two pronuclei.

Day 2 after the puncture
The fertilised egg has already developed to a two to four-cell group. Now we decide whether to put the embryo(s) back into the female uterus (embryo transfer) on day 3 or 5. You will receive a phone call from the KinderWunschKlinik laboratory today to make the date for embryo transfer!

Day 3 after the puncture

The fertilised egg may have already divided itself into an eight-cell group. Either we insert it (them) into the female uterus today or we wait until day 5.

Day 4 after the puncture
The morula stage is reached.

Day 5 after the puncture
The embryo(s) is (are) in the blastocyst stage and is (are) inserted back into the uterus today at the latest. This procedure is completely pain-free, similar to a gynaecological examination and requires no anaesthesia. The patient is asked to arrive at the KinderWunschKlinik with a full bladder!

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